Even before Silent Spring was published by Houghton Mifflin in 1962, there was strong opposition to it. According to Time in 1999:

Carson was violently assailed by threats of lawsuits and derision, including suggestions that this meticulous scientist was a "hysterical woman" unqualified to write such a book. A huge counterattack was organized and led by Monsanto, Velsicol, American Cyanamid, indeed, the whole chemical industry duly supported by the Agriculture Department as well as the more cautious in the media.

In the 1960s, biochemist and former chemical industry spokesman Robert White-Stevens stated, "If man were to follow the teachings of Miss Carson, we would return to the Dark Ages, and the insects and diseases and vermin would once again inherit the earth."

Industry and agribusiness advocates continue to criticize Silent Spring. In a 2005 essay, "The Harm That Pressure Groups Can Do", British politician Dick Taverne was damning in his criticism of Carson:

Carson didn't seem to take into account the vital role (DDT) played in controlling the transmission of malaria by killing the mosquitoes that carry the parasite (...) It is the single most effective agent ever developed for saving human life (...) Rachel Carson is a warning to us all of the dangers of neglecting the evidence-based approach and the need to weight potential risk against benefit: it can be argued that the anti-DDT campaign she inspired was responsible for almost as many deaths as some of the worst dictators of the last century.

However, DDT has never been banned for anti-malaria use, and Carson argued in Silent Spring that:

No responsible person contends that insect-borne disease should be ignored. The question that has now urgently presented itself is whether it is either wise or responsible to attack the problem by methods that are rapidly making it worse. The world has heard much of the triumphant war against disease through the control of insect vectors of infection, but it has heard little of the other side of the story—the defeats, the short-lived triumphs that now strongly support the alarming view that the insect enemy has been made actually stronger by our efforts. Even worse, we may have destroyed our very means of fighting. ... What is the measure of this setback? The list of resistant species now includes practically all of the insect groups of medical importance. ... Malaria programmes are threatened by resistance among mosquitoes. ... Practical advice should be 'Spray as little as you possibly can' rather than 'Spray to the limit of your capacity' ..., Pressure on the pest population should always be as slight as possible.

In the 2000s, Carson and Silent Spring have come under increasing attack from authors who argue that restrictions placed on DDT have caused needless death, and more generally that environmental regulation unnecessarily restricts economic freedom. For example, the conservative magazine Human Events gave Silent Spring an "honorable mention" in its list of the "Ten Most Harmful Books of the 19th and 20th Centuries," and in 2002, to mark its 40 anniversary, Reason Magazine published an essay by economist Ronald Bailey, a former fellow with the libertarian Competitive Enterprise Institute. Bailey argued that the book had a mixed legacy:

The book did point to problems that had not been adequately addressed, such as the effects of DDT on some wildlife. And given the state of the science at the time she wrote, one might even make the case that Carson's concerns about the effects of synthetic chemicals on human health were not completely unwarranted. Along with other researchers, she was simply ignorant of the facts. But after four decades in which tens of billions of dollars have been wasted chasing imaginary risks without measurably improving American health, her intellectual descendants don't have the same excuse.

Some environmentalists consider this latter day criticism of Silent Spring and Rachel Carson and concomitant push for DDT to be an industry sponsored strategy to discredit the environmental movement. For example, Monica Moore of Pesticide Action Network has argued that "Renewed promotion of DDT and attacks on those who would limit its use isn’t about malaria, or even DDT. It is a cynical 'better living through chemistry' campaign intended to discredit the environmental health movement, with support from the Bush administration and others who seek nothing less than the dismantling of health and environmental protections."